Cipher: 2306
Nomenclature: Free radicals, lipid peroxidation and cell growth control
Study programme: Molecular biosciences
Module: Biology
Case holder:

title doc. dr.sc. Morana Jaganjac, zn. Counsellor
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:

the doc.dr.sc. Lidija Milković, zn. Associate
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Understand the role of free radicals and products of lipid peroxidation in controlling the growth of normal and malignant cells.
Case contents:

Pathophysiology of oxidative stress - genotoxicity
Lipid peroxidation and bioactive reactive aldehydes as other messengers of free radicals
Creation of DNA assets with lipid peroxidation products
Initiation of lipid peroxidation using electromagnetic radiation and photodynamic stress
Methodology of lipid peroxidation – direct and indirect methods for the determination of lipid hydroperoxides and reactive aldehydes
Oxidation of proteins, proteosomes and adducts of 4-hydroxynonenala and proteins – biochemical and medical consequences
4-hydroxynonnenal (HNE) - signaling pathways and elimination
HNE metabolism in subcellular fractions, cells, organs and the human body
Molecular bases of malignant transformation, role (proto)oncogenes, suppressor genes and regulatory cytokines – similarities and differences in signal transmission between normal and malignant cells
Modulation of growth by products of lipid peroxidation with special emphasis on hne
Mediators of oxidative stress in necrosis and apoptosis, cellular adaptation to oxidative stress
Yeasts as a model for controlling cell growth under oxidation stress
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Analyze the role of free radicals and lipid peroxidation products in controlling the growth of normal and malignant cells.
2. Critically evaluate the relevant scientific literature dealing with the study of oxidative stress.
3. Propose new hypotheses on the dependence of free radicals and lipid peroxidation products in controlling the growth of normal and malignant cells.
4. Propose a methodology for examining new hypotheses and highlight the importance of such research.
ECTS Credits 6
Lectures 5
Seminars (IS) 5
Exercises (E) 20
Altogether 30
The way of teaching and acquiring knowledge:

regular attendance with possible justified absence of up to 2 hours of classes
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance
Rating method: Written exam
Mandatory literature:

Eckl, P.M., Ortner, A. and Esterbauer, H. (1993) GENOTOXIC PROPERTIES OF 4-HYDROXYALKENALS AND ANALOGOUS ALDEHYDES. Mutation Res. 290, 183-192.
Chung et al., LIPID PEROXIDATION AS A POTENTIAL SOURCE FOR THE FORMATION OF EXOCYCLIC DNA ADDUCTS, Carcinogenesis, 1996, 17, 2105-2111
Žarković N. Žarković K. Schaur RJ. Stolc S. Schlag G. Redl H. Waeg G. Loncaric I. Jurić G. Hlavka V. 4-HYDROXYNONENAL AS A SECOND MESSENGER OF FREE RADICALS AND GROWTH MODIFYING FACTOR. Life Sci, 1999;65: 1901-1904
Aust S.D. LIPID PEROXIDATION. In: (Greenwald R.A. ed) Handbook of Methods for Oxygen Radical Research. CRC Press, Boca Raton, FL, 1985; Pp. 203-207.
Supplementary (recommended) literature:

Girotti A.W. PHOTOSENSITIZED OXIDATION OF MEMBRANE LIPIDS: REACTION PATHWAYS, CYTOTOXIC EFFECTS, AND CYTOPROTECTIVE MECHANISMS. J. Photochem. Photobiol. B: Biology, 63 (2001) 103-113.
Wonisch W., Kohlwein S.D., Schaur J., Tatzber F., Guttenberger H., Zarkovic N., Winkler R., Esterbauer H. TREATMENT OF THE BUDDING YEAST SACCHAROMYCES CEREVISIAE WITH THE LIPID PEROXIDATION PRODUCT 4-HNE PROVOKES A TEMPORARY CELL CYCLE ARREST IN G1 PHASE. Free Radic Biol Med 1998; 25:682-687
Biasi F., Tessitore L., Zanetti D., Citrin J.C., Zingaro B., Chiarpotto E., Žarković N., Serviddio G., Poli G. ASSOCIATED CHANGES OF LIPID PEROXIDATION AND TGF1 LEVELS IN HUMAN CANCER DURING TUMOR PROGRESSION. Gut, 50: 361-367, 2002
How to monitor the quality and performance performance (evaluation):

For individual lectures – thematic units - verification of understanding of the information obtained, the method of presentation, etc.
Discussions with students and colleagues - survey upon completion of the course.
Tracking each student's progress.
Evaluation of performance by the head of studies and the joint expert committee of the institutions of the holders of the study. The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek."