Cipher: 2505
Nomenclature: Molecular mechanisms of oxidative stress in plants
Study programme: Molecular biosciences
Module: Plant biology
Case holder:

Prof.dr.sc. Tihana Teklić
Prof.dr.sc. Hrvoje Lepeduš
Institution of the case holder:

(TT) Faculty of Agrobiotechnical Sciences in Osijek
(HL) Faculty of Humanities and Social Sciences, Josip Juraj Strossmayer University of Osijek
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

To know the mechanisms of oxidative stress formation at the molecular, subcellular and cellular levels, and the mechanisms of elimination of the factors that cause it, with special emphasis on the abiotic factors of the environment.
Case contents:

Oxygen and reactive oxygen individuals. Damage to biomolecules and cellular structures in conditions of oxidative stress. Oxidants and signal conduction in the cell. Nonenzyme antioxidants: ascorbic acid, glutathione, vitamin E, carotenoids, phenols. Antioxidant enzymes: catalase, peroxidases, superoxide dismutase, glutathione reductase and monodehydroaskorbat reductase. Halliwell-Asada cycle. Abiotic factors of oxidative stress: sub- and supraoptimal temperatures, water affordability, soil salinity, deficit and feed surplus, contamination of the soil with heavy metals.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Analyze fundamental theoretical knowledge from the biology of oxidative stress.
2. Explain the mechanisms of stress tolerance in plants.
3. Predict mechanisms for the formation and elimination of oxidative stress with respect to a particular abiotic environmental factor.
4. To assess the optimal mechanisms of the formation and elimination of oxidative stress with regard to the interaction of various abiotic environmental factors.
5. Identify modern instrumental techniques used in plant stress research.
ECTS Credits 6
Lectures 25
Seminars (IS) 5
Exercises (E) 0
Altogether 30
The way of teaching and acquiring knowledge:

Students are obliged to attend lectures or consultations, and prepare and exhibit seminar work
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral exam, Essay/Seminar, Publication Analysis, Project, 9
Mandatory literature:

BUCHANAN, B., GRUISSEM, W., JONES, R. L., 2015: Biochemistry and Molecular Biology of Plants. 2nd ed., American Society of Plant Biologists, Wiley Blackwell.
HANCOCK, J. T., 2008: Redox-mediated Signal Transduction. Methods and Protocols. Humana Press.
SCHEEL, D., WASTERNACK, C., 2003: Plant Signal Transduction. Oxford University Press, New York.
TAIZ, L., ZEIGER, E. (2006): Plant Physiology. 4th Edition. Sinauer Associates, Inc.
Supplementary (recommended) literature:

BEGOVIĆ, LYDIA; MLINARIĆ, SELMA; ANTUNOVIĆ DUNIĆ, JASENKA; KATANIC, ZORANA; LONCARIC, ZDENKO; LEPEDUŠ, HRVOJE; CESAR, VERA. Response of Lemna minor L. to short-term cobalt exposure: The effect on photosynthetic electron transport chain and induction of oxidative damage. Aquatic toxicology. (2016) ; 175; 117–126.
ANTUNOVIĆ DUNIĆ, JASENKA; LEPEDUŠ, HRVOJE; ŠIMIĆ, DOMAGOJ; LALIĆ, ALOYSIUS; MLINARIĆ, SELMA; KOVACEVIC, JOSIP; CESAR, VERA. Physiological response to different irradiation regimes during barley seedlings growth followed by drought stress under non-photoinhibitory light. Journal of agricultural science. 7 (2015) , 6; 69-83.
ŠIMIĆ, DOMAGOJ; LEPEDUŠ, HRVOJE; JURKOVIĆ, VLATKA; ANTUNOVIĆ, JASENKA; CESAR, VERA. Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments. Journal of integrative plant biology. 56 (2014), 7; 695-708.
KOVACEVIC, JOSIP; KOVACEVIC, MAJA; CESAR, VERA; DREZNER, GEORGE; LALIĆ, ALOYSIUS; LEPEDUŠ, HRVOJE; ZDUNIĆ, ZVONIMIR; JURKOVIĆ, ZORICA; DVOJKOVIĆ, KREŠIMIR; KATANIC, ZORANA; KOVACEVIC, VLADO. Photosynthetic efficiency and quantitative reaction of bread winter wheat to mild short-term drought conditions. Turkish journal of agriculture and forestry. 37 (2013) ; 385-393.
RADIC, SANDRA; PEHAREC ŠTEFANIĆ, PETRA; LEPEDUŠ, HRVOJE; ROJE, VIBOR; PEVALEK-KOZLINA, BRANKA. Salt tolerance of Centaurea ragusina L. is associated with efficient osmotic adjustment and increased antioxidative capacity. Environmental and experimental botany. 87 (2013) ; 39-48.
LELJAK-LEVANIĆ, DUNJA; JEZIC, MARIN; CESAR, VERA; LUDWIG-MÜLLER, JUTTA; LEPEDUŠ, HRVOJE; MLADINIC, MARIN; KATIC, MARIJA; ĆURKOVIĆ-PERICA, MIRNA. Biochemical and epigenetic changes in phytoplasma-recovered periwinkle after indole-3-butyric acid treatment. Journal of applied microbiology. 109 (2010) , 6; 2069-2078.
GILL, S.S., TUTEJA, N. (2010): Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry. 48(12): 909-930.
KRANNER, I., BECKETT, R.P., MINIBAYEVA, F.V., SEAL, C.E. Tansley review: What is stress? Concepts, definitions and applications in seed science. New Phytologist 2010;188:655-673.
PUCIARIELLO, C., BANTI, V., PERATA, P. (2012): ROS signaling as common element in low oxygen and heat stresses. Plant Physiology and Biochemistry. 59: 3-10.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.