Cipher: 2312
Nomenclature: Molecular control of hemotopoiesis and leukemogenesis
Study programme: Molecular biosciences
Module: Biology
Case holder:

the prof.dr.sc. Mariastefania Antica, zn. Advisor-permanent choice
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Understand the investigations of the differentiation and development of hematopoietic nut cells to mature immunocompetent lymphocytes and molecular mechanisms and verification points during this process.
Case contents:

Molecular control of cell differentiation from the starting stem cells on the hematopoiesis or lymphopoesis model. Stem cells as units responsible for the development, restoration and maintenance of tissues and organic systems. Genetically modified, transgenic, mice: the technology of studying the function of genes; importance in basic and medical research. Hematopoietic stem cells and bone marrow transplantation on an experimental mouse model. Transcription control of genes in lymphocytes. The molecular basis of lymphocyte maturation, the signals that promote gene reshuffle, and the delay in lymphocyte development in leukemias and lymphomas. Apoptosis – the life and death of cells.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Set the hypothesis and accordingly the methodology of research into the differentiation and development of hematopoietic stem cells.
2. Carry out the planned research.
3. Interpret the results of the research.
4. Conclude on the basis of the results obtained and anticipate the contribution of the conducted research in the overall understanding of molecular control of hematopoiesis and leukogenesis.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 10
Altogether 20
The way of teaching and acquiring knowledge:
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Mandatory seminar work
Rating method: Written exam, Oral exam
Mandatory literature:

1. Immunobiology. The immune system in health and disease, Janeway Travers
2. Molecular Cell Biology, by Lodish Berk, Matsudaira, Kaiser, Krieger, Scott, Zipursky, Darnell,
Supplementary (recommended) literature:

1. L. Wu, M. Antica G. R. Johnson, R. Scollay and K. Shortman (1991) Developmental potential of the earliest precursor cells from the adult mouse thymus. J.Exp. Honey 174, 1617 - 1627
2.M. Antica B. Kusic, R. Spaventi, B. Jaksic and B. Vitale (1993) Functional differences of T cells in B Chronic Lymphocytic Leukemia. Leukemia and Lymphoma 9, 133-140
3.M. Antica L. Wu, K. Shortman and R. Scollay (1993) Intrathymic lymphoid precursor cells during fetal thymus development. J. Immunol. 151, 1-9
4.C. L. Li, L.Wu, M. Antica K. Shortman, and G. R. Johnson (1994) Purified murine long-term in vivo hemopoietic repopulating cells are not pro-thymocytes. Exp. Haematol 23, 21-25
5.M. Antica L. Wu, K. Shortman and R. Scollay (1994) Thymic stem cells in the mouse bone marrow. Blood 84, 111-117
6. J. Ismaili, M. Antica and L. Wu (1996) CD4 and CD8 expression and T cell antigen receptor gene rearrangement in early intrathymic precursor cells. Eur. J. Immunol. 26 731-737.
7.M. Antica L. Wu, K. Shortman and R. Scollay (1997) Stem cell antigen 2 (Sca-2) expression in adult and developing mice. Immunol. Letters 55 47-51.
8.M. Antica, R. Scollay (1999) Development of T lymphocytes at extrathymic sites. J. Immunol., 163 206-211.
9. Antica M. Kusic B. Hranilovic D. Dietz AB. Vuk-Pavlovic S. Cloning the cDNA for murine U2 snRNP-A ' gene and its differential expression in lymphocyte development. [Article] Immunology Letters. 82(3):217-223, 2002.
10. Vitale B. Martinis M. Antica M. Kusic B. Rabatic S. Gagro A. Kusec R. Jaksic B. Prolegomenon for chronic lymphocytic leukaemia [Review]. [Review] Scandinavian Journal of Immunology. 58(6):588-600, 2003c.
11. Kusic B. Dominis M. Dzebro S. Antica M. Molecular insight into the diagnosis of lymphoma. [Article] International Journal of Molecular Medicine. 12(4):667-671, 2003.
How to monitor the quality and performance performance (evaluation):

Consultations and seminars