Cipher: 2315
Nomenclature: Metabolomics
Study programme: Molecular biosciences
Module: Biology
Case holder:

the doc.dr.sc. Morana Lamb, zn. Counsellor
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:

the doc.dr.sc. Matea Nikolac Perković, zn. Associate
the doc.dr.sc. Gordana Nedić Erjavec, zn. Associate
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

To learn the specific aspects of metabolomics as an essential branch of the so-called "omics" of analytical scientific fields and to understand the importance of interdisciplinary nature in comprehensive biomedical research.
Case contents:

- Interdisciplinary nature of biomedical research
- Omics approaches
- Metabolism and generation of metabolites
- Specifics of metabolomic research
- Basic methods of metabolomics, preparation and analysis of samples, analysis and interpretation of results
- Targeted and general approaches, complementarities of methods
- Mass spectrometry - the specifics and possibilities of liquid and gas chromatography
- "Targeted" and "untargeted" metabolomics
- Use of metabolomics
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Critically interpret the results of publications in the field of metabolomics.
2. Apply an appropriate approach to metabolomic research.
3. Analyse new data from available databases in areas -omics, in particular metabolomics.
4. Predict the use of metabolomics in other scientific fields and disciplines.
ECTS Credits 6
Lectures 5
Seminars (IS) 5
Exercises (E) 20
Altogether 30
The way of teaching and acquiring knowledge:

Regular attendance with possible justified absence of up to 4 hours
Ways of teaching and acquiring knowledge: (notes)

Students will be able to see the work of the new metabolomic unit within the LabOS of the Rudjer Boskovic Institute, and the specifics of scientific cooperation with clinicians and foreign partners.
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities
Rating method: Viva voce
Mandatory literature:

1. Patti GJ et al (2012) Metabolomics: the apogee of the omics trilogy. Nat Rev Mol Cell Biol. 2012 Mar 22,13(4):263-9.
2. Agnieszka Gęgotek, Jacek Nikliński, Neven Žarković, Kamelija Žarković, Georg Waeg, Wojciech Łuczaj, Radosław Charkiewicz, Elżbieta Skrzydlewska (2016) Lipid mediators involved in the oxidative stress and antioxidant defence of human lung cancer cells. Redox Biology, 9:210-219
3. Luka Andrisic, Danuta Dudzik, Coral Barbas, Lidija Milkovic, Tilman Grune, Neven Zarkovic (2018) Short overview on metabolomics approach to study pathophysiology of oxidative stress in cancer. Redox Biology 14: 47-58
4. Nedic Erjavec G, Konjevod M, Nikolac Perkovic M, Svob Strac D, Tudor L, Barbas C, Grunec T, Zarkovic N, Pivac N (2018) Short overview on metabolomic approach and redox changes in psychiatric disorders. Redox Biology 14: 178-186
Supplementary (recommended) literature:

1. Al-Menhali AS, Banu S, Angelova PR, Barcaru A, Horvatovich P, Abramov AY, Jaganjac M. Lipid peroxidation is involved in calcium dependent upregulation of mitochondrial metabolism in skeletal muscle. Biochim Biophys Acta Gen Subj. (2019); 1864(3):129487.
2. Morana Jaganjac, Shamma Almuraikhy, Fatima Al-Khelaifi, Mashael Al-Jaber, Moataz Bashah, Nayef A. Mazloum, Kamelija Zarkovic, Neven Zarkovic, Georg Waeg, Wael Kafienah (2017) Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients. Redox Biology 12: 483-490
3. Ambrożewicz, Ewa; Wójcik, Piotr; Wroñski, Adam; Łuczaj, Wojciech; Jastrząb, Anna; Zarkovic, Marigold; Skrzydlewska, Elzbieta (2018) Pathophysiological Alterations of Redox Signaling and Endocannabinoid System in Granulocytes and Plasma of Psoriatic Patients. Cells. 7. 159. 10.3390/cells7100159
4. Marcela Konjevod, Lucija Tudor, Dubravka Svob Strac, Gordana Nedic, Erjavec, Coral Barbas, Neven Zarkovic, Matea Nikolac Perkovic, Suzana Uzun, Oliver Kozumplik, Gordan Lauc, Nela Pivac (2019) Metabolomic and glycomic findings in posttraumatic stress disorder. Prog Neuro-Psychopharmacol Biol Psychiatry, 88:181-193
5. Al-Thani AM, Voss SC, Al-Menhali AS, Barcaru A, Horvatovich P, Al Jaber H, Nikolovski Z, Latiff A, Georgakopoulos C, Merenkov Z, Segura J, Alsayrafi M, Jaganjac M. Whole blood storage in CPDA1 blood bags alters erythrocyte membrane proteome. Oxid Med Cell Longev. 2018 (2018); 6375379.
6. Wishart S. Emerging applications of metabolomics in drug discovery and precision medicine. Nat Rev Drug Discov. 2016 Jul;15(7):473-84.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.