Cipher: 2308
Nomenclature: Genomic DNA
Study programme: Molecular biosciences
Module: Biology
Case holder:

home doc.dr.sc.Vesna Musani, higher zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Learn the properties of genomic DNA, methods and purpose of isolation.
Case contents:

Introduction to genomic DNA properties, isolation methods, detection and analysis methods: from polymerase chain reaction (PCR), loss of heterozygity, single-stranded sequence analysis (SSCP) and heteroduplex, and sequencing. The use of genomic maps and databases to analyze coding and non-coding parts of the genome.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Critically judge databases.
2. Analyze genes and sequences.
3. Design beginners and set chain reactions with polymerase.
4. Conclude on the basis of an analysis of the results.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 10
Altogether 20
The way of teaching and acquiring knowledge:

regular attendance in all forms of teaching, active participation, knowledge of internet use
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities
Rating method: Oral exam, Case review, Continuous knowledge check in the course of classes
Mandatory literature:

Sambrook J, Fritsch EF, Maniatis T (1989) Isolation of high molecular weight DNA from mammalian Cells. In: Cold Spring Harbor Laboratory Press (ed.) Molecular cloning - a laboratory manual, 9.14-23.
HUGO consortium (2001) The human genome. Science 291: 1145-1434.
Human genomes. (2001) Nature 409:745-964
Supplementary (recommended) literature:

1. Glavač D, Dean M (1993) Optimization of the Single Strand Conformation Polymorphism (SSCP) Technique for detection of Point Mutations. Hum Mut 2:404-414
2. Danaee T, Nelson HH, Karagas MR, Schned AR, Ashok TDS, Hirao T, Perry AE, Kelsey KT (2002) Microsatellite instability at tetranucleotide repeats in skin and cancer bladder. Oncogene21:4894-4899.
3. Phelan CM, Larsson C, Baird S, Futreal AP, Ruttledge MH, Morgan K, Tonin P, Hung H, Korneluk RG, Pollak MN, Narod SA(1996) The human mammary-derived growth inhibitor gene (MDGI):genomic structure and mutation analysis in human breast cancer. Genomics 34:63-68.
4. Watson JD, Gilman M, Witkowski J, Zoller M.(1991) Recombinant DNA. Sci Am books , W H Freeman and comp New York
5. Glavač D, Dean M (1995) Applications of heteroduplex Analysis for Mutation Detection in Disease genes. Human Mut 6: 281-287.
6. Griffiths AJE, Miller JH, Suzuki DT, Lewontin RC (1992) An introduction to genetic analysis, 5th edd. WH Freeman and comp New York
7. Johnson DR, Levanat S, Bale AE. (1995) Direct Molecular Analysis of Archival Tumor Tissue
8. for Loss of Heterozygosity. BioTechniques 19(2):190 -192.
9. Shimkets R, Gailani M, Siu V, Yang-Feng T, Pressman C, Levanat S, Goldstein A, Dean M, Bale
10. AE. (1996) Molecular Analysis of Chromosome 9q Deletions in Two Gorlin Syndrome Patients.
11. Am J Hum Genet 59:417-422.
12. Levanat S, Chidambaram A,Wicking C, Bray-Ward P, Pressman C,Toftgard R, Gailani MR, Wainwright B, Dean M, Bale AE. (1997) Pulsed-Field Gel Electrophoresis and FISH Mapping of Chromosome 9q22: Placement of a Novel Zinc Finger Gene within the NBCCS and ESS1 region. Cytogenet Cytogenet Cell Genet 76:208-213.
How to monitor the quality and performance performance (evaluation):

Poll
The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.