Cipher: 2506
Nomenclature: Herbal toxicity tests
Study programme: Molecular biosciences
Module: Plant biology
Case holder:

Prof.dr.sc. Janja Horvatić
Institution of the case holder:

Department of Biology, University of Josip Juraj Strossmayer in Osijek
Contributors - Contractors:

Doc.dr.sc. Martina Varga
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Learn the action of a known factor on the test organism in laboratory conditions, as well as its possible impact on the living world in the environment.
Case contents:

Types of tests. Test organisms. How to perform tests in the laboratory. An individual representation of the toxicity of metals and xenobiotics to algae, which, by their toxicity and/or representation in industry or environment, deserve special attention. The origin and quantity of xenobiotics in water. Solder test - as indicators of toxicity, plant increment is most often monitored (by determining fresh and dry matter, determining the total area of plants), the concentration of photosynthetic pigments and the amount of proteins. Determination of metal toxicity (lead, cadmium, mercury, manganese, chromium, nickel, cobalt, and aluminum) and xenobiotics on the algae cult. Chlorella kessleri, Pseudokirchneriella subcapitata and Desmodesmus subspicatus using the miniaturized bioassay method. Statistical data processing.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Apply an appropriate herbal toxicity test.
2. Monitor the selected toxicity indicators in a particular type of test.
3. Critically interpret experimentally collected data.
4. To investigate the effects of a particular toxicant on plant organisms in relevant literature sources.
5. Identify potential environmental problems that can be solved through the use of acquired knowledge.
ECTS Credits 6
Lectures 20
Seminars (IS) 5
Exercises (E) 5
Altogether 30
The way of teaching and acquiring knowledge:

Regular attendance of lectures with possible justified absence up to 2 hours, regular attendance of exercises without the possibility of absence.
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral exam, Publication Analysis, Practical paper
Mandatory literature:

OECD GUIDELINES FOR THE TESTING OF CHEMICALS- Revisedproposal for a newguideline 221Lemna sp. GrowthInhibition Test (http://www.oecd.org/dataoecd/16/51/1948054.pdf
HOCK B., ELSTNER E.F., 2004. Plant Toxicology, CRC Press
Rai, L.C., Gaur, J.P. & Soeder, C.J., 1994: Algae and water polution. E. Schweizerbart, Stuttgart.

GARCIA-LORENZO, M.L., MARTINET-SÁNCHEZ, M. J., PÉREZ-SIRVENT, C., 2014: Application if a plant bioassay for the evaluation of ecotoxicological risks of heavy metals in sediments affected by mining activities. Journal of Soils and Sediments, Volume 14, Issue 10
(http://link.springer.com/article/10.1007/s11368-014-0942-0)

PARK, A., KIM, Y.-J., CHOI, E.-M., BROWN, M. T., HAN, T., 2013: A novel bioassay using root re-growth in Lemna. Aquatic Toxicology. Volume 140-141
(http://www.sciencedirect.com/science/article/pii/S0166445X13001471)
Supplementary (recommended) literature:

MKANDAWIRE, M., TEIXEIRA DA SILVA, J.A., GERT DUDEL, E., 2014: The Lemna Bioassay: Contemporary issues as the most standardized plant bioassay for aquatic ecotoxicology. Critical Reviews in Environmental Science and Technology, Volume 44, Issue 2
(http://www.tandfonline.com/doi/full/10.1080/10643389.2012.710451)

VISIOLI, G., CONTI, F.D., GARDI, C., MENTA C., 2014: Germination and root elongation bioassays in six different plant species for testing Ni contamination in soil. Bulletin of Environmental Contamination and Toxicology, Volume 92, Issue 4
(http://link.springer.com/article/10.1007%2Fs00128-013-1166-5)

SANDÍN-ESPAÑA, P., LAUREIRO, I., ESCORIAL, C., CHUECA, C., SANTÍN-MONTANYÁ, I., 2011: The bioassay technique in the study of the herbicide effects. IN: SOLONESKI, S., LARRAMENDY, L.M., (Ed.): Herbocodes, Theory and Applications; InTech
(http://www.intechopen.com/books/herbicides-theory-and-applications/the-bioassay-technique-in-the-study-of-the-herbicide-effects)

NAUMANN, B., EBERIUS, M., APPENROTH, K. J., 2007: Growth rate based dose – response relationships and EC-values of ten heavy metals using the duckweed growth inhibition test(ISO 20079) with Lemna minor L. clone St. Journal of Plant Physiology, Volume 164, Issue 12
(http://www.sciencedirect.com/science/article/pii/S0176161706003154)

LANDOLT, E., 1986: The family of Lemnaceae – a monographic study (Vol. 1 and 2). Veroffentlichungen des Geobotanischen Institutes der Edig. Tech. Hochschule, StiftungRübel. Zurich.
How to monitor the quality and performance performance (evaluation):

- checking the understanding of the information received, the method of presentation
- discussions with students and colleagues.
- monitoring the progress of each student
- "the success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek"