Cipher: 2303
Nomenclature: Supramolecular structures and oxidative stress
Study programme: Molecular biosciences
Module: Biology
Case holder:

Dr. sc. Suzana Šegota, Scientific Advisor
Prof.dr.sc. Hrvoje Lepeduš
Institution of the case holder:

(HL) Faculty of Humanities and Social Sciences, Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Introduce students to cell membranes as a supramolecular structure, and to their roles in lipid peroxidation and the formation of atherosclerosis and malignancies as well as spectroscopic and magnetic-resonance methods in the study of oxidative stress.
Case contents:

Cell membrane as a supramolecular structure: organization of building elements (asymmetry of the lipid two-layer, lipid-protein interaction, rafts) and dynamic properties (fluidity, liquid-crystal state). Transport through the membrane. The role of the membrane in the oxidation process. Atherosclerosis and malignancies. Liposomes and their use in oncology. Basic spectroscopic methods in the investigation of oxidation processes of in-vitro (Uv-Vis, infrared spectroscopy, fluorescence). Application of magnetic resonance imaging (NMR and EPR) methods in detecting free radicals and tissue oxygenation in-vivo and ex-vivo.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain basic theoretical knowledge about magnetic-resonant, spectroscopic and fluorometric methods.
2. Review the use of magnetic-resonant, spectroscopic and fluorometric methods in the study of oxidative stress.
3. Assess the roles of biomembranes in malignancies and oxidative stress.
4. Propose possible solutions to improve the study of oxidative stress and the role of biomembranes in malignancies.
ECTS Credits 4
Lectures 15
Seminars (IS) 5
Exercises (E) 0
Altogether 20
The way of teaching and acquiring knowledge:
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Mandatory seminar paper
Rating method: Viva voce
Mandatory literature:

1. T. McKee and J.R. McKee: Biochemistry,Wm.C. Brown publishers, 1996.
2. H. Esterbauer, J. Gebicki, H. Puhl, G. Juergens: The role of lipid peroxidation and antioxidants in oxidative modification of LDL, Free radic. Biol. Honey. 13 (1992) 341-390.
3. A.Giessauf, E. Steiner, H. Esterbauer: Early destruction of tryptophan residues of apolipoprotein B is a vitamin E-ependent process during copper-mediated oxidation of LDL, Biochem. The biophys. Acta 1256 (1995) 221-232.
4. H. Kalaji et al.: Frequently asked questions about in vivo chlorophyll fluorescence: practical issues. Photosynth. Res. 122 (2014) 121-158.
Supplementary (recommended) literature:

S. Banuelos, J.L. Arrondo, F.M. Goni, G. Pifat: Surface-core relationship in human low density lipoproteins as studied by infrared spectroscopy, J. Biol. Chem. 270 (1995) 9192-9196.
Bioradicals detected by ESR spectroscopy, (H. Ohya-Nishiguchi, L. Packer eds.), Birkaeuser Verlag, 1995
G.K.E. van Holde, W.C. Johnson, P.S. Ho: Principles of physical biochemistry, Prentice Hall, Upper Saddle River, USA, 1998
How to monitor the quality and performance performance (evaluation):

For individual lectures – thematic units - verification of understanding of the information obtained, the method of presentation, etc.
Discussions with students and colleagues - survey upon completion of the course.
Tracking each student's progress.
Evaluation of performance by the head of studies and the joint expert committee of the institutions of the holders of the study. The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek."