Cipher: 1105
Nomenclature: Application of cell and tissue cultures in biomedical research
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

home doc.dr. Sc. Suzana Borović Šunjić, senior zn. Associate
Dr. sc. Josipa Vlainić, Senior Research Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

Master basic methods of cell and tissue cultivation and methods of monitoring basic cell growth parameters to enable independent and responsible work with cell cultures.
Case contents:

- advantages and disadvantages of the use of cell cultures; biology of cell cultures
- aseptic methods and maintaining sterility
- conditions for growing cell cultures: substrates, medium and temperature
- isolation of primary cell cultures
- cultivation of organoculture
- maintenance and preservation of cell cultures
- measurement of viability and cytotoxicity
- design of experiments and interpretation of results
- specialized techniques of working with cell cultures
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the basics of growing cells and working with cell cultures.
2. Manage the work in sterile conditions.
3. Independently design an experiment involving work with cell cultures.
4. Critically interpret the results of a self-designed experiment.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 15
Altogether 25
The way of teaching and acquiring knowledge:

regular attendance with possible justified absence of up to 2 hours of classes
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance
Rating method: Written exam
Mandatory literature:

1. Sović A, Borović S, Loncaric I, Kreuzer T, Žarković K, Vuković T, Waeg G, Hrašćan R, Wintersteiger R, Klinger R, Zurak N, Schaur RJ, Žarković N: The carcinostatic and proapoptotic potential of 4-hydroxynonenal in HeLa cells is associated with its conjugation to cellular proteins. Anticanc Res, 9:1997-2004, 2001
2. Semlitsch T, Tillian HM, Žarković N, Borović S, Purtscher M, Hohenwarter O, Schaur RJ: Differential Influence of the Lipid Peroxidation Product 4-Hydroxynonenal on the Growth of Human Lymphatic Leukaemia Cells and Human Peripheral Blood Lymphocytes. Anticancer Res. 10:1689-1697, 2002
3. Wildburger R, Žarković N, Borović S, Žarković K, Kejla Z: Enhanced osteogenesis: Systemic consequence of traumatic brain injury: U: Shock, sepsis and Organ Failure, 5th Wiggers Bernard Conference. Schlag G, Redl H, Traber DL, (your.), Springer-Verlag, Heidelberg, 305-318, 1997
4. Freshney RI: Animal cell culture: A practical approach, Irl Press
Script 5 (in preparation)
Supplementary (recommended) literature:

1. Freshney RI: Culture of animal cells, Wiley-Liss
2. Adams R.L.P.: Laboratory techniques in biochemistry and molecular biology, ELSEVIER
3. Borović Šunjić, Suzana; Lace, Ana; Wildburger, Renate; Žarkovic, Marigold. The influence of 4-hydroxy-2-nonenal on proliferation and differentiation of human osteosarcoma cells. Biofactors. 2005, in print
How to monitor the quality and performance performance (evaluation):

For individual lectures – thematic units - verification of understanding of the information obtained, the method of presentation, etc.
Discussions with students and colleagues - survey upon completion of the course.
Tracking each student's progress.
Evaluation of performance by the head of studies and the joint expert committee of the institutions of the holders of the study. The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek."