Basic Methods in Molecular Biology and Medicine (1115)

home assoc.prof.dr. Sc. Andreja Ambriović Ristov, zn. Counsellor
Assoc. prof.dr. sc. Neda Slade, zn. Counsellor

Ruđer Boskovic Institute

the doc.dr.sc. Dragomira Majhen, higher zn. Associate
the doc.dr.sc. Angela Horvat, zn. Associate
the doc.dr.sc. Ignatius is a member, zn. Associate

Acquire theoretical and practical knowledge about basic methods of work in biology and medicine.

The subject is a set of lectures and exercises that cover the most important methods in experimental work in biology and medicine. Most methods also include exercises performed in the laboratory. Each lecturer in the subject begins with a theoretical introduction, so that students can follow the practical work further. Continuous enrichment of the content of objects with modern methods is foreseen. The seminars jointly cover the articles of the lecturers themselves with the aim of explaining why and for what purpose a particular method is used.
The case involves practical work, however, some methods due to the length of duration and/or price, are processed only by demonstration. Continuous enrichment of the content of objects with modern methods is foreseen. The seminars jointly cover the articles of the lecturers themselves with the aim of explaining why and for what purpose a particular method is used.
The subject includes the following methods: DNA and RNA isolation, electrophoresis in agarous gel, polymerase chain reaction (PCR), reverse transcription (RT), sodium dodecil sulfate electrophoresis (SDS-PAGE), Western blot, analysis of PCR splitting reaction products with the help of restrictive enzymes (RFLP), immunocytochemistry, cell cultures, transfection, determination of apoptosis (fluorescence, DNA fragment isolation), cloning into plasmid vector, Southern and dot hybridization, real time PCR, PCR, determination of apoptosis (fluorescence, DNA fragment isolation), cloning into plasmid vector, Southern and dot blot hybridization, real time PCR, PCR, determination of primary sequence of nucleotide-sequencing, heteroduplex analysis, analysis of heterozygoticity loss analysis, analysis of single-stranded DNA forms, gene libraries, confocal laser search microscopy, electron microscopy, Ames test, gel electrophoresis in variable electric field (PFGE), biochemical methods of protein purification, DNA microarray.

1. Explain the principles of the basic methods of molecular biology.
2. Critically judge the relevance of the materials and methods used in articles in the field of molecular biology with regard to the research task/objective set.
3. Independently plan and write the chapter Materials and methods in a scientific article and doctoral dissertation.

Regular attendance of lectures and active participation in seminars and exercises.

Initially, the exercises will be performed in the laboratories of lecturers, but over time we hope that the Rudjer Boskovic Institute will provide a smaller laboratory intended for this very purpose. This course is based on three years of experience of the educational project "METHODOLOGICAL COURSES IN BIOLOGY AND MEDICINE" organized within the Rudjer Boskovic Institute www.metode.avalon.hr

Methods in molecular biology, Ambriović Ristov A., Brozović A., Bruvo Mađarić B., Cetković H., Herak Bosnar M., Hranilovic D., Katušić Hećimović S., Meštrović Radan N., Mihaljević S., Slade N., Vujaklija D. (editors). Ruđer Boskovic Institute, Zagreb, 2007.

Select the latest articles from literature; changes every year.

The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek, and the leaders will receive information from the participants about the adequacy of the program and performance by the leadership through the survey.