Cipher: 1106
Nomenclature: Basics of immunochemical methods
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

Assoc. prof.dr. sc. Paško Konjevoda, zn. adviser
Institution of the case holder:

Rudjer Boskovic Institute, Zagreb
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

See the benefits of immunochemical analyses in qualitative and quantitative characterizations of very small amounts of substances directly in complex biological samples and recognize immunochemical methods as sensitive, simple, selective, rapid and cost-effective replacements or additions to available analytical methods
Case contents:

Definition of antigen (epitope) and antibodies (paratope). Antigen-antibody reactions (specificity, affinity, cross reactivity) and the basics on which immunochemical techniques are based. Immunization and preparation of specific polyclonal antibodies: immunogenics (cells, large molecules, haptens, preparation of haptene conjugates), immunization (animals, immunization procedures, adjuvants), classes of immunoglobulin, collection and storage of antiserums. Preparation of monoclonal and recombinant antibodies. Isolation, purification and characterization of antibodies. Marking antibodies.
Immunothalization, agglutination, inhibition of agglutination. Methods of deposition in the gel in conditions of diffusion (single and double, single- and two-dimensional immunodiffusion). Electrophoresis and immunoelectrophorese, buging and its immunodetecence. Radiochemical Methods (RIA). Immunoenzymatic (ELISA) and immunofluorescent methods (FIAs). Flow cytometry.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the basics of the use of immunochemical methods in biomedicine.
2. Critically assess relevant scientific literature in the field of immunochemistry.
3. Critically judge the relevance of data and facts from other sources of information in the field of immunochemistry.
4. To judge the appropriateness of a particular imuochemical method in solving an independently recognised and specific problem in the field of immunochemistry.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 15
Altogether 25
The way of teaching and acquiring knowledge:

Regular attendance, preparation of seminar work, participation in practical classes, discussions
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work, Exercise or case study
Rating method: Written exam, Oral exam, Essay/Seminar, Continuous examination of knowledge in the course of teaching
Mandatory literature:

Turgeon ML. Immunology & Serology in Laboratory Medicine. 5th edition. 2014, Amsterdam, Elsevier.
Supplementary (recommended) literature:

Wild D (editor). The Immunoassay Handbook. 4th edition. 2013, Amsterdam, Elsevier.
Dodig S. Immunochemistry. 2015, Medical Publishing, Zagreb.
How to monitor the quality and performance performance (evaluation):

Verification of the understanding of the information received for thematic units.
Examining the adequacy of the program, the way of presentation, teaching materials, the adoption of material at the end of the lecture.
Discussions with students and colleagues.
Tracking each student's progress.
The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek.