Cipher: 2501
Nomenclature: Organization and function of plant cells
Study programme: Molecular biosciences
Module: Plant biology
Case holder:

Prof.dr.sc. Hrvoje Lepeduš
Institution of the case holder:

Faculty of Humanities and Social Sciences, Faculty of Dental Medicine and Health, University of Josip Juraj Strossmayer in Osijek
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

To understand the molecular structure of plant cells, the dynamic connection of the organization of cellular structures and biomolecules with their function, and to understand the continuity of cellular processes.
Case contents:

The significance and function of chemical bonds in interactions within biological systems. Organization and chemism of the biomembrane. Transport through the biomembrane. Cell cycle and cytoskeleton. Structure and function of the interphase nucleus: chromosomes, DNA and genes. Replication. Transcription. Mitosis and meiosis. Endoplasmic reticulum, ribosomes and protein biosynthesis. Golgi system, lysosomes, peroxisomes, glioxsisomes, vacuoles. Mitochondria: ultrastructure and function. Plasticides and plastic pigments. Ultrastructure chloroplast. Molecular organization of thylacoid membranes: photosynthetic pigments, photoshoot systems, electron-transport chain. Calvin's cycle. Photosynthesis and evolution.
In the practice, experimental techniques in the research of photosynthesis will be processed: chromatographic and spectrophotometric analysis of photosynthetic pigments, determination of net-photosynthesis using oxygen electrode, in vivo measurement of chlorophyll fluorescence by the saturatic pulse method.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Analyze the association between the organization and the functions of plant cells.
2. Propose ways to visualize cell events to determine the connection between cellular structures and their function.
3. Critically assess the pros and cons of some modern laboratory techniques used in plant cell research.
4. Critically judge the methodology presented in the scientific literature that leads to new scientific knowledge about the structure and function of plant cells.
ECTS Credits 6
Lectures 25
Seminars (IS) 5
Exercises (E) 0
Altogether 30
The way of teaching and acquiring knowledge:

Students are obliged to attend lectures or consultations, all practice and seminars.
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral exam, Publication analysis, Continuous verification of knowledge in the course of teaching, 9, Practical work
Mandatory literature:

BUCHANAN, B., GRUISSEM, W., JONES, R. L., 2015: Biochemistry and Molecular Biology of Plants. 2nd ed., American Society of Plant Biologists, Wiley Blackwell.
LEPEDUŠ, H., CESAR, V., 2010: Basics of plant histology and anatomy of vegetative organs. J. J. Strossmayer University of Osijek, Department of Biology, Osijek.
ALBERTS, A., JOHNSON, A., LEWIS, J., RAFF, M., ROBERTS, K., WALTER, P., 2007: Molecular biology of the cell. 5th ed. Garland Science, New York - Abingdon.
HELDT, H.–W., 1999: Plant Biochemistry and Molecular Biology. Oxford University Press Inc., New York, Oxford.
Supplementary (recommended) literature:

VOJTA, LEAH; CARIĆ, DEJANA; CESAR, VERA; ANTUNOVIĆ DUNIĆ, JASENKA; LEPEDUŠ, HRVOJE; KVEDER, MARINA; FULGOSI, HRVOJE. TROL-FNR interaction reveals alternative pathways of electron partitioning in photosynthesis. Scientific reports. 5 (2015):10085, DOi: 10.1038/srep10085.
FULGOSI, H., LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N. Differential accumulation of plastid preprotein translocon components during spruce (Picea abies L. Karst.) needle development. Biol. Chem., 386 (2005), 777-783.
CESAR, V., LEPEDUŠ, H., LJUBEŠIĆ, N. Histochemical observations on the needles of Norway spruce (Picea abies L. Karst.) trees affected by cement dust pollution. Phyton, 44 (2004), 205-217.

LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N. Chloroplast ultrastructure and chlorophyll levels in vegetative buds and needles of Norway spruce (Picea abies L. Karst.). Period. Biol.,103 (2001), 61-65.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.