Molecular Mechanisms of Immunopathophysiological Response in Systemic Inflammatory Response and Sepsis (2616)

Prof. dr.sc Jerko Barbić
Prof. dr.sc. Ines Drenjančević

Faculty of Medicine, Josip Juraj Strossmayer University of Osijek

Understand the molecular mechanism of the systemic reactions of the organism to tissue damage or infection that leads to systemic inflammatory response syndrome and sepsis. To recognize these mechanisms as the basis of new therapeutic procedures in the treatment of this complex and in a large number of cases of the deadly condition.

Definition of concepts: systemic inflammatory response syndrome (SIRS), sepsis, septic shock, multisystem organ failure syndrome (MOFS of multiple organ failure syndrome).
Molecular characteristics of the most common causative agents of sepsis (virulent factors and adhesion molecules) and interaction with the host's immune system. PAMP (pathogen associated molecular patterns) such as LPS, lipoteicoic acid, peptidoglycans and DNA germs and their role. Molecular mechanisms of recognition of PAMPs and internal hazard signals in tissue damage (the role of TLR from engl toll like receptors, C-reactive protein and lectin as molecules that recognize PAMP).
Principles of excitation of nonspecific and specific immunity (the role of macrophages, lymphocytes). The role of inflammatory cytokines (TNF-α, IL-1, IL-8) and anti-inflammatory cytokines (IL-10, TGF-β). Congenital deficiencies of nonspecific immunity (complement, phagocytes and natural antibodies) in the onset of sepsis. Molecular mechanisms of control of the inflammatory response in sepsis (the role of LBP, soluble CD14 receptor, soluble IL-1 and TNF-α receptors). Allele gene polymorphism for TNF-α and IFN-γ and sepsis severity.
The role of microcirculation in pathogenesis and pathophysiology of sepsis: mechanisms of damage and dysfunction of the endothelium as the main mechanism of the formation of multiorgan failure and shock (the role of NO, bradykinin, PAF). Sepsis and mechanisms of hemodynamic changes. Mechanisms of the formation of disseminated intravascular coagulation (DIK).
Clinical indicators SIRS, sepsis and MOFS. Modern therapeutic procedures that can act on the pattern of immune response in sepsis.

1. Assess the relevant methods for conducting experiments on an in vitro model of sepsis.
2. Explain the basics of activation of the immune system (congenital and specific immunity), in particular the role of molecular patterns related to pathogens and tissue damage.
3. Identify mechanisms for hemodynamic instability in patients in the intensive care unit.
4. Critically review relevant sources and information related to the occurrence of septic collapse and their contribution to the understanding of the development of new therapeutic procedures.
5. Assess which therapeutic procedures may affect the pattern of immune response in sepsis.

regular attendance and preparation for classes,

Editor: Abbas AK. Cellular and Molecular Immunology, 8th Edition 2014

PDF versions of current review articles in the area.

The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek.