Cipher: 1111
Nomenclature: Molecular spectroscopy of biological systems
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

the izv.prof.dr.sc. Vlasta Mohaček Grošev, visa zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

Understand the possibilities of applying molecular spectroscopies, especially Raman and infrared spectroscopy in the study of biological systems.
Case contents:

Theoretical basics: how the organism, the tissue, the cell... we detect vibrational spectroscopies; a description of the inherent states of molecules, which are allowed transitions in molecules in Raman and infrared spectroscopy, which spectral interval describes the collective excitations of multiple molecules, whether we always perceive it and by what method; order and disorder in complex systems.

Practical exercises: Study of several selected simple systems (organic molecules in a liquid state, piece of tissue or organism) in laboratory exercises. Each student gets his own system and problem to solve and with the help of a mentor writes a seminar paper that he orally presents.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Examine the principles of quantum physics and chemistry on which spectroscopic methods (Ramanova, infrared, UV-VIS, EPR and NMR) are based.
2. Apply vibrational spectroscopies to the analysis of molecule dynamics.
3. Analyze the selected solutions or powder using the Ab initio program Gaussian and the method of normal modes.
4. Discuss the development of the SERS technique in biomedicine for the identification of nanomolar concentrations in the solution.
5. Assess relevant literature sources and interpret scientific data critically.
ECTS Credits 6
Lectures 5
Seminars (IS) 5
Exercises (E) 25
Altogether 35
The way of teaching and acquiring knowledge:

Mandatory attendance of lectures, work in the laboratory and preparation of an independent task.
Ways of teaching and acquiring knowledge: (notes)

The course is carried out in the Laboratory for Molecular Physics of the Department of Material Physics of the Rudjer Boskovic Institute
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Mandatory seminar work
Rating method: 9, Practical work
Mandatory literature:

1.V. Mohaček Grošev: Application of vibrational spectroscopy to biological systems, 2016 script
2.P. Matousek: Emerging Raman Applications and Techniques in Biomedical and Pharmaceutical Fields, Springer 2010.
3. Frank S. Parker: Applications of Infrared Spectroscopy in Biochemistry, Biology and Medicine, Plenum Press 1971.
Supplementary (recommended) literature:

R. A. Nyquist: Interpreting Infrared, Raman and Nuclear Magnetic Resonance Spectra, Vol1., Academic Press 2001.
How to monitor the quality and performance performance (evaluation):

The success of the selection of material and interpretation will be accompanied by questionnaires distributed to students before and after the course. How much the subject has met expectations will be evaluated by the head of the studies, and every year the success of the course will be evaluated by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek.