Cipher: 1114
Nomenclature: Models and methods for the investigation of oxidative stress in carcinogenesis
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

the doc.dr.sc. Ana Čipak Gašparović, senior zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

Learn which models are used to investigate oxidative stress in carcinogenesis and understand the methods and tests that are applicable to individual models.
Case contents:

- animal and cellular models
- methods for determining reactive oxygen compounds
- methods for determining the antioxidant mechanisms of cell defense
- methods for determining lipid peroxidation products
- methods for determining the cytotoxic and genotoxic effects of oxidative stress
- methods for determining the effects of oxidative stress on proteins
- methods for determining the effects of oxidative stress on the genome
- commercially available tests to determine oxidative stress
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the molecular aspects and regulation of oxidative stress in the development of tumors.
2. Examine the role of oxidative stress and lipid peroxidation in carcinogenesis.
3. Anticipate problems in choosing a model for oxidation stress research.
4. Apply the right approach to assessing the optimal model choice for targeted research.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 15
Altogether 25
The way of teaching and acquiring knowledge:

Students are obliged to attend classes regularly with a possible justifiable absence of up to 2 hours of classes
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral exam, Essay/Seminar
Mandatory literature:

1. Borović Šunjić S, Lace A, Rabuzin F, Wildburger R, Žarković N (2005) The Influence of 4-Hydroxy-2-nonenal on Proliferation, Differentiation and Apoptosis of Human Osteosarcoma Cells. Biofactors 24, 141-148
2. Methods in Molecular Biology (Andrea Ambriović Ristov, Chief Your.), Rudjer Boskovic Institute, Zagreb 2007
3. Pork A, Lamb M, Tehlivets O, Kohlwein SD, Zarkovic N. Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast. Biochimica et Biophysica Acta 1781 (2008); 283-287.
4. Gasparović AC, Jaganjac M, Mihaljević B, Šunjić SB, Žarković N, Assays for the measurement of lipid peroxidation. In: Methods in molecular biology (Clifton NJ your.), Humana Press, 2013, 965:283-296.
5. Frijhoff, J; Winyard, PG; Zarkovic, N; Davies ,SS; Stocker, R; Cheng, D; Knight, AR; Taylor, EL; Oettrich, J; Ruskovskaya, T; Pork Gasparovic, A; Cuadrado,A; Weber, D; Enghusen Poulsen, H; Grune, T; Schmidt, HHW; Ghezzi P. (2015) Clinical Relevance of Biomarkers of Oxidative Stress, Antioxid Redox Signal. Doi: 10.1089/ars.2015.6317
Supplementary (recommended) literature:

1. Marquez-Quiñones, Adriana, Ana Cipak, Kamelija Zarkovic, Fattel-Fazenda, S., Villa-Treviño S., Neven Zarkovic and Guéraud, F. (2009) HNE-protein adducts formation in different pre-carcinogenic stages of hepatitis in LEC rats. Free Radical Research, (2010), 44 (2):119-127
2. Gueraud F, Atalay M, Bresgen N, Cipak A, Eckl PM, Huc L, Jouanin I, Siem W, Uchida K, Chemistry and biochemistry of lipid peroxidation products Free Radical Research, 2010, 44(10):1098-1124.
3. Žarković N, Lace A, Jaganjac M, Borović S, Žarković K, Pathophysiological relevance of aldehydic protein modifications. J Proteomics. 2013 30;92:239-47
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.