Cipher: 1117
Nomenclature: Microtechnics and microscopy methods
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

Prof.dr.sc. Branko Dmitrović
Doc. dr. sc. Jasenka Antunović Dunić
Institution of the case holder:

(BD) Faculty of Dental Medicine and Health, J.J. Strossmayer University of Osijek
(JAD) Department of Biology, J.J. Strossmayer University
Contributors - Contractors:

Doc.dr.sc. Jasenka Antunović Dunić
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

Master the knowledge and skills of preparing cytological and histological plant and human preparations as well as methods of microscopy.
Case contents:

Introduction to plant microtechnics. Sampling of plant material. Introduction to human microtechnics and obtaining samples. Fixation. Dehydration. Infiltration and fitting in. The use of microtoms. Histochemical and cytochemical reactions: fresh slits, cuts incorporated into paraffin, methacrylate and epoxy resins. Immunolocalization. In situ hybridization of nucleic acids. Light microscopy. Types of light microscopes: microscope with phase and differential-interference contrast, fluorescence microscope, confocal microscope. Electron microscopy: TEM and SEM (ESEM).

During the practice, students will independently make cytological and histological preparations, paint and microscopy, and learn to analyze the results obtained.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the ways of taxidermy and observation of cellular structures and tissues.
2. Assess appropriate fixation techniques and further tissue taxidermy.
3. Adequately use the methods of light and fluorescent microscopy.
4. Analyze independently made preparations suitable for the desired research.
5. Make a photodocumentation.
6. Critically judge the prerequisites for valid scientific reasoning in the analysed scientific papers.
ECTS Credits 4
Lectures 15
Seminars (IS) 6
Exercises (E) 5
Altogether 26
The way of teaching and acquiring knowledge:

Students are obliged to attend lectures or consultations, and all practicums.
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Teaching activities, exercise or case study
Rating method: Oral exam, Analysis of published publication, Continuous verification of knowledge in the course of teaching, Practical work
Mandatory literature:

RUZIN, S.E., 1999: Plant Microtechnique and Microscopy. Oxford University Press, New York, Oxford.
MALIGA, P., KLESSIG, D. F., CASHMORE, A. R., GRUISSEM, W., VARNER, J. E., 1995: Methods in Plant Molecular Biology. A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, New York.
O'BRIEN, T. P., MCCULLY, M. E., 1981: The Study of Plant Structure. Princples and Selected Methods. Termercarphi Pty. Ltd., Melbourne, Australia.
WILKINSON, D. G., 1998: In situ Hybridization. Practical Approach. Oxford University Press, Oxford, New York, Tokyo.
AMBRIOVIĆ RISTOV, A., BROZOVIĆ, A., BRUVO MAĐARIĆ, B., CETKOVIĆ, H., HERAK BOSNAR, M., HRANILOVIC, D., KATUŠIĆ HEĆIMOVIĆ, S.,MIHALJEVIĆ S., SLADE, N.,VUJAKLIJA, D. (editors): Methods in molecular biology, Rudjer Boskovic Institute, Zagreb, 2007.
JUNQUEIRA, C.L., CARNEIRO, J. Basics of histology: textbook and atlas. Zagreb: Medical publishing 2005.
Supplementary (recommended) literature:

FULGOSI, H., LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N., 2005: Differential accumulation of plastid preprotein translocon components during spruce (Picea abies L. Karst.) needle development. Biol. Chem., 386, 777-783.
LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N., 2005: Photosystem II efficiency, chloroplast pigments and fine structure in previous-season needles of Norway spruce (Picea abies L. Karst.) affected by urban pollution. Period. Biol., 107(3), 329-333.
CESAR, V., LEPEDUŠ, H., LJUBEŠIĆ, N., 2004: Histochemical observations on the needles of Norway spruce (Picea abies L. Karst.) trees affected by cement dust pollution. Phyton, 44, 205-217.
LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N., HAS-SCHÖN, E., 2003: Photosynthetic pigments, chloroplast distribution and fine structure in vegetative buds of two spruce species. Biologia (Bratislava), 58, 867-873.
LEPEDUŠ, H., CESAR, V., LJUBEŠIĆ, N., 2001: Chloroplast ultrastructure and chlorophyll levels in vegetative buds and needles of Norway spruce (Picea abies L. Karst.). Period. Biol., 103, 61-65.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.