Cipher: 2512
Nomenclature: Tissue culture and genetic transformation of plants
Study programme: Molecular biosciences
Module: Plant biology
Case holder:

the doc.dr.sc. Snježana Mihaljević, senior zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

To know the application of plant cell and tissue culture, and genetic engineering of commercially important cultures. Understand the correlation between basic and applied research in agronomy and forestry, and the advantages and disadvantages of breeding genetically modified plant species.
Case contents:

Vegetative reproduction in in vitro conditions. Culture of cells, protoplasts and calluses. Anther culture. Plant growth regulators. Transformation of plant cells with special attention to transform using A. tumefaciens and A. rhizogenes. Genetically modified plant species. Resistance to pathogens, herbicides, etc. Biological control of plant diseases by genetic transformation of pathogens. Horizontal gene transfer in vitro and in vivo.

Practicum: vegetative reproduction of shoots infected with plant pathogens, proving transgenes in plant tissue or proving pathogens.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Assess the possibility of applying knowledge about plant tissue culture in plant biotechnology.
2. Propose the use of a method for micropropagation of plant material of interest.
3. Analyze the principles of genetic engineering on plants.
4. Master transgene intake and detection techniques in plant cells.
5. Critically assess the risks of horizontal gene transfer based on relevant data and information.
ECTS Credits 4
Lectures 5
Seminars (IS) 5
Exercises (E) 10
Altogether 20
The way of teaching and acquiring knowledge:

Students are obliged to attend lectures, practice and seminars.
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral exam, Essay/Seminar, Continuous examination of knowledge in the course of teaching, 9, Practical work
Mandatory literature:

Ausubel, FM et al. Current Protocols in Molecular Biology. Greene Pub. Associates; Wiley-Interscience, New York, 2012.
Jelaska S. Culture of plant cells and tissues. School book, Zagreb, 1994.
Sambrook J. Russel D. Molecular cloning: a laboratory manual, 3rd ed. CSH Laboratory press, Cold Spring Harbor, 2001.
Supplementary (recommended) literature:

Ambriović-Ristov A, Brozović A, Bruvo Mađarić B, Cetković H, Hranilovic D, Herak Bosnar M, Katušić Hećimović S, Meštrović Radan N, Mihaljević S, Slade N, Vujaklija D. Molecular methods in biology. Ruđer Boskovic Institute, Zagreb, 2007.
Hull R, Tzotzos GT, Head. Genetically Modified Plants: Assessing Safety and Managing Risk. Academic Press, London, 2009.
Poppy GP, Wilkinson MJ. Gene Flow from GM Plants. John Wiley & Sons, NewYork, 2008.
Smith RH. Plant Tissue Culture: Techniques and Experiments, 3rd edition. Academic Press, London 2013.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.