Cipher: 2302
Nomenclature: Immunogenic transplantation
Study programme: Molecular biosciences
Module: Biology
Case holder:

the prof.dr.sc. Zorana Grubić, zn. Advisor-permanent choice
home doc.dr. Sc. Renata Žunec, zn. Counsellor
Institution of the case holder:

KBC-Zagreb
Contributors - Contractors:

doc.dr.sc. Katarina Štingl Janković
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Introduce students to the HLA system and its role in tissue and organ transplantation as well as with the latest scientific knowledge and their application in transplant immunogenetics.
Case contents:

The main system of tissue matching in man – Human Leukocyte Antigens (HLA): a historical overview of discoveries, research, terminology, accommodation and structure. Biological role of the HLA system: processing and presentation of peptides. The structure of HLA grade I and Grade II HLA genes and molecules.
The basic genetic characteristics of the HLA system: polymorphism, phenotype, haplotype, genotype,
association imbalance – linkage disequilibrium, recombination-crossing over. Laboratory tests for determination of genes, antigens and antibodies HLA: MLCT, PCR-SSP, PCR-SSO, sequencing. Selection of organ recipients for cadaveral organ transplantation. Choosing a donor for hematopoietic cell transplantation. The role of HLA gene matching and the choice of typification methods in tissue and organ transplantation. The role of HLA (PRA) antibodies in tissue and organ transplantation.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the basics of immunogenetics and transplantation of tissues and organs.
2. Critically read literature in the field of transplant immunology.
3. To determine the molecular and cellular aspects and regulation of the transplant reaction.
4. Critically extract relevant data and information from scientific databases for the purpose of researching the role of the HLA gene in tissue and organ transplantation.
ECTS Credits 4
Lectures 10
Seminars (IS) 10
Exercises (E) 0
Altogether 20
The way of teaching and acquiring knowledge:
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Mandatory seminar work
Rating method: Written exam, Essay/Seminar
Mandatory literature:

1)Andreis I, Batinić D, Čulo F, Grčević D, Marušić M, Taradi M, Višnjić D. Immunology. Zagreb: Medical Publishing, 2004; 2)Apperley J.F., Gluckman E., Gratwohl A. Blood and marrow transplantation. Paris: European School of Haematology, 2000. 3)Blair GE, Pringle CR, Maudesley DJ. Modulation of MHC antigen expression and disease. Cambridge: University Press, 2005. 4)Cecka J.M., Terasaki P.I. Clinical Transplants 2003. Los Angeles: UCLA Tissue typing Laboratory, 2004. 5)Janeway C.A., Travers P., Walport M., Shlomchik M.J. Immunobiology 5, The Immune system in health and disease. New York: Garland Publishing, 2001. 6)Marsh S.G.E., Parham P., Barber L.D. The HLA facts book. London: Academic Press, 2000.
Supplementary (recommended) literature:

Magazines; Human Immunology, Clinical Transplantation, Tissue Antigens, Transplantation Proceedings
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.