Cipher: 2108
Nomenclature: Genetic engineering in biotechnology
Study programme: Molecular biosciences
Module: Materials science and new technologies
Case holder:

home doc.dr. Sc. Michaela Matovina, zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:

the doc.dr.sc. Branka Salopek Sondi, zn. Advisor-permanent choice
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Master theory and application of recombinant DNA technology and train students to research and work in biotechnology.
Case contents:

Prokaryotic and eukaryotic DNA: differences in the mechanisms of regulation of gene activity. Nuclear and extranuclear DNA. DNA fragmentation: restrictive nucleases, restrictive mapping. Separation of DNA molecules by size: gel electrophoresis. DNA sequencing: chemical splitting, dideoxy method. Hybridization of nucleic acids in vitro and in situ. DNA cloning: plasmid and viral vectors. Purpose and application of recombinant DNA technology: gene substitution in bacteria and lower eukaryotes, creation of specific dominant mutants in diploid organisms, production of transgenic organisms, gene therapy.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. To examine the role of molecular biology in genetic engineering and biotechnology.
2. Analyze the latest genetic engineering techniques.
3. Explain modern laboratory techniques used in the creation and analysis of transgenic organisms.
4. Assess the possible application of the latest developments in biotechnology in the field of own professional or scientific activity.
ECTS Credits 6
Lectures 10
Seminars (IS) 5
Exercises (E) 15
Altogether 25
The way of teaching and acquiring knowledge:

Regular teaching and active participation – regular preparation of reports on the exercises performed and interpretation of results based on knowledge acquired in theoretical and practical teaching
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Exercise or case study
Rating method: Oral exam, Publication Analysis, Practical paper
Mandatory literature:

NICHOLL D.S.T. , 2008: An introduction to genetic engineering, 3rd ed. Cambridge University Press, New York
DELIĆ, V., 1997: Genetic engineering in biotechnology. PMF, Zagreb.
Supplementary (recommended) literature:

VOET, D., VOET J.G., 2004: Biochemistry, 3rd ed. John Wiley & Sons, Inc. York.
BERG, J.M., TYMOCZKO, J.L., STRYER, L., 2002: Biochemistry, 5th ed. Freeman & Co, New York.
FOSTER, G.D., TWELL, D., 1996: Plant gene isolation: principles and practice. John Wiley&Sons, Chicester- New York.
HUGES, M.A., 1996: Plant molecular genetics. Longman, Edinburgh gate, Harlow.
TALARO K. P. 2005 Foundations in microbiology: basic principles, 5th ed. McGrow-Hill, New York.
LEWIS R. 2003 Human genetics: concepts and applications, 5 th ed. McGrow-Hill, New York.
HSU P.D., LANDER, E.S., ZHANG F., 2014, Development and Applications of CRISPR-Cas9 for Genome Engineering. Cell 157:1262-1278.
DOUDNA J.A., CHARPENTIER E., 2014, Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096.
How to monitor the quality and performance performance (evaluation):

By talking to students and anonymous surveys.
The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.