Cipher: 2301
Nomenclature: Biology of tumor and normal cells
Study programme: Molecular biosciences
Module: Biology
Case holder:

the doc.dr.sc. Maya Sabol, zn. Associate
Institution of the case holder:

Ruđer Boskovic Institute
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester II
Subject objective:

Understand the latest findings on the normal functioning of cells of multicellular organisms, the regulation of the cell cycle, the transmission of signals in the cell and between cells, and the disruption of these processes with molecularly genetic and biochemical aspects of cell functioning and the research model in vitro and in vivo.
Case contents:

Course content: cell structure and cell cycle, protein manifestation in cell cycle regulation, growth factors and protooncogens, signal transmission, gene activation and inactivation, gene map, recessive and predominantly hereditary diseases. Apoptosis and blockers of apoptosis. Phosphorylation and defosphorylation - phosphatases and kinases and their inhibitors.
Methods of molecular biology: isolation of proteins and nucleic acids, detection methods (chromatographic, immunochemical, electrophoretic), radioactive and fluorescent marking of fragments and/or cells, polymerase chain reaction, various types of hybridizations (northern, western, dot blot, southern, in situ, substractic and differential); research models: cell lines, paraffin incisions, primary cell cultures, laboratory animals, fresh tissue.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Predict the genetic and protein role in the functioning of the cell and the molecular regulation of cell survival.
2. Assess the relevance of different sources of literature and data on molecular physiology and functioning of cells/organisms for the purpose of researching the development of the disease.
3. Critically interpret scientific data and, on that basis, offer new hypotheses for research into the development of the disease.
4. Valorize possible methods and approaches in investigations of cell functioning.
5. Solve specific problems and situations in the working environment by applying the knowledge gained.
ECTS Credits 6
Lectures 5
Seminars (IS) 5
Exercises (E) 20
Altogether 30
The way of teaching and acquiring knowledge:

attendance of classes and exercises, critical analysis of the scientific article
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Oral Exam, Published Publication Analysis
Mandatory literature:

Lodish H et al Molecular Cell Biology, 7th edd,2012;

Pelengaris S, Khan M. The molecular biology of cancer: a bridge from bench to bedside, New York : Wiley, 2013
Locker J. Transcription Factors.2001. BIOS Scientific Publ Lim Oxford,OX4 1RE, UK
Supplementary (recommended) literature:

Alberts B,Johnson A,Lewis J,Morgan D,Raff M, Roberts K, Walter P Molecular biology of the cell, 6th edd. 2014

Ambriović Ristov, A et al in Methods in Molecular Biology; University of Zagreb and Rudjer Boskovic Institute in 2007
How to monitor the quality and performance performance (evaluation):

Survey among students
The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.