Cipher: 1112
Nomenclature: Analysis of the locus of quantitative properties
Study programme: Molecular biosciences
Module: elective course (methodological)
Case holder:

The headline izv.prof.dr.sc. Domagoj Šimić, zn. advisor-permanent choice
Izv.prof.dr.sc. Sonja Petrović
Institution of the case holder:

(DŠ) Agricultural Institute Osijek
(SP) Faculty of Agrobiotechnical Sciences, Josip Juraj Strossmayer University of Osijek
Contributors - Contractors:
Subject status: Electoral College
The year in which the case is submitted: Year I
The semester in which the case is submitted: Semester I
Subject objective:

Understand the analysis of the locus of quantitative properties (QTL) as a statistical method of combining phenotypic (measurable properties) and genotypic data (molecular markers) to resolve the genetic basis of varying complex properties in plants and animals and in medicine.
Case contents:

Quantitative (complex) properties. Phenotyping and genotyping. Genetic mapping. Population types for QTL mapping. The number of loci and genes. Population size. Analysis of individual markers, interval and composite interval mapping, association mapping. Analysis of concrete data and cases. Overview of computer programs for QTL analysis. Genomic resources. Reference populations. From QTL to genes: positional cloning, candidate genes. Mapping and microchips, expressive QTLs. Future trends.
Learning outcomes: competences, knowledge, skills that the subject develops:

1. Explain the basis of genetic analysis of complex properties.
2. Link phenotypic and genotypic (molecular) data for a specific quantitative property.
3. Apply quantitative genetic analyses for quantitative (measuring) properties.
4. Critically select relevant literature sources and scientific data based on the genetics of quantitative properties.
5. Plan and perform research to solve problems from the real working environment.
ECTS Credits 4
Lectures 15
Seminars (IS) 5
Exercises (E) 5
Altogether 25
The way of teaching and acquiring knowledge:

Students are obliged to attend lectures or consultations, and to prepare and exhibit seminar work.
Ways of teaching and acquiring knowledge: (notes)
Monitoring and evaluating students (mark in fat printing only relevant categories) Attendance, Teaching activities, Mandatory seminar work
Rating method: Written exam, Oral exam, Essay/Seminar, Analysis of published publication, 9
Mandatory literature:

HEDRICK, P.W., 2000. Quantifying QTLs. U: Genetics of populations. Jones and Bartlett Publishers, Boston, pp 493-499.
MILES, C., WAYNE, M., 2008. Quantitative trait locus (QTL) analysis. Nature Education 1(1). Available from: http://www.nature.com/scitable/topicpage/quantitative-trait-locus-qtl-analysis-53904
SHADT, E.E., MONKS, S.A., DRAKE, T.A., LUSIS, A.J., CHE, N., COLINAYO V., RUFF, T.G., MILLIGAN, S.B., LAMB, J.R., CAVET, G., LINSLEY, P.S., MAO, M., STOUGHTON, R.B., FRIEND, S.H. Genetics of gene expression surveyed in maize, mouse and man. Nature, 422, 297-302.
WAYNE, M.L:, MCINTYRE, L.M., 2002. Combining mapping and arraying: An approach to candidate gene identification. Proceedings of the National Academy of Sciences, 99, 14903-14906.
Supplementary (recommended) literature:

ANDORF, C.M., LAWRENCE, C.J., HARPER. LC, SCHAEFFER ML, CAMPBELL YES, SEN Z, 2010. The Locus Lookup tool at MaizeGDB: identification of genomic regions in maize by integrating sequence information with physical and genetic maps. Bioinformatics, 26, 434-436.
KEARSEY, M.J., 1998. The principles of QTL analysis (a minimal mathematics approach). Journal of Experimental Botany, 49, 327, 1619-1623.
ŠIMIĆ, D., MLADENOVIĆ DRINIĆ, S., ZDUNIĆ, Z., JAMBROVIĆ, A., LEDENČAN, T., BRKIĆ, J., BRKIĆ, A., BRKIĆ, I., 2012. Quantitative trait loci for biofortification traits in maize grain. Journal of Heredity, doi: 10.1093/jhered/esr122.
How to monitor the quality and performance performance (evaluation):

The success of the course will be evaluated annually by the joint expert committee of the Ruđer Boskovic Institute, the University of Dubrovnik and the University of Josip Juraj Strossmayer in Osijek based on exam success and surveys.