Metabolomika (2315)

naslovna doc.dr.sc. Morana Jaganjac, zn. savjetnica

Institut Ruđer Bošković

naslovna doc.dr.sc. Matea Nikolac Perković, zn. suradnica
naslovna doc.dr.sc. Gordana Nedić Erjavec, zn. suradnica

Naučiti specifične aspekte metabolomike kao bitne grane tzv. -omics analitičkih znanstvenih područja te spoznati važnost interdisciplinarnosti u sveobuhvatnim biomedicinskim istraživanjima.

- Interdisciplinarnost biomedicinskih istraživanja
- Omics pristupi
- Metabolizam i generacija metabolita
- Specifičnosti metabolomskih istraživanja
- Osnovne metode metabolomike, priprema i analiza uzoraka, analiza i interpretacija rezultata
- Ciljani i općeniti pristupi, komplementarnosti metoda
- Masena spektrometrija – posebnosti i mogućnosti tekućinske i plinske kromatografije
- „Targeted“ i „untargeted“ metabolomika
- Primjena metabolomike

1. Kritički interpretirati rezultate publikacija iz područja metabolomike.
2. Primijeniti odgovarajući pristup metabolomskih istraživanja.
3. Analizirati nove podatke iz dostupnih baza podataka u područjima -omics, posebice metabolomike.
4. Predvidjeti primjenu metabolomike u drugim znanstvenim područjima i disciplinama.

Redovito pohađanje nastave uz mogući opravdani izostanak do 4 sata

Studenti će biti u prilici vidjeti rad nove metabolomske jedinica u okviru LabOS-a Instituta Ruđer Bošković, te specifičnosti znanstvene suradnje s kliničarima i inozemnim partnerima.

1. Patti GJ et al (2012) Metabolomics: the apogee of the omics trilogy. Nat Rev Mol Cell Biol. 2012 Mar 22;13(4):263-9.
2. Agnieszka Gęgotek, Jacek Nikliński, Neven Žarković, Kamelija Žarković, Georg Waeg, Wojciech Łuczaj, Radosław Charkiewicz, Elżbieta Skrzydlewska (2016) Lipid mediators involved in the oxidative stress and antioxidant defence of human lung cancer cells. Redox Biology, 9:210-219
3. Luka Andrisic, Danuta Dudzik, Coral Barbas, Lidija Milkovic, Tilman Grune, Neven Zarkovic (2018) Short overview on metabolomics approach to study pathophysiology of oxidative stress in cancer. Redox Biology 14: 47-58
4. Nedic Erjavec G, Konjevod M, Nikolac Perkovic M, Svob Strac D, Tudor L, Barbas C, Grunec T, Zarkovic N, Pivac N (2018) Short overview on metabolomic approach and redox changes in psychiatric disorders. Redox Biology 14: 178-186

1. Al-Menhali AS, Banu S, Angelova PR, Barcaru A, Horvatovich P, Abramov AY, Jaganjac M. Lipid peroxidation is involved in calcium dependent upregulation of mitochondrial metabolism in skeletal muscle. Biochim Biophys Acta Gen Subj. (2019); 1864(3):129487.
2. Morana Jaganjac, Shamma Almuraikhy, Fatima Al-Khelaifi, Mashael Al-Jaber, Moataz Bashah, Nayef A. Mazloum, Kamelija Zarkovic, Neven Zarkovic, Georg Waeg, Wael Kafienah (2017) Combined metformin and insulin treatment reverses metabolically impaired omental adipogenesis and accumulation of 4-hydroxynonenal in obese diabetic patients. Redox Biology 12: 483-490
3. Ambrożewicz, Ewa; Wójcik, Piotr; Wroński, Adam; Łuczaj, Wojciech; Jastrząb, Anna; Zarkovic, Neven; Skrzydlewska, Elzbieta (2018) Pathophysiological Alterations of Redox Signaling and Endocannabinoid System in Granulocytes and Plasma of Psoriatic Patients. Cells. 7. 159. 10.3390/cells7100159
4. Marcela Konjevod, Lucija Tudor, Dubravka Svob Strac, Gordana Nedic, Erjavec, Coral Barbas, Neven Zarkovic, Matea Nikolac Perkovic, Suzana Uzun, Oliver Kozumplik, Gordan Lauc, Nela Pivac (2019) Metabolomic and glycomic findings in posttraumatic stress disorder. Prog Neuro-Psychopharmacol Biol Psychiatry, 88: 181-193
5. Al-Thani AM, Voss SC, Al-Menhali AS, Barcaru A, Horvatovich P, Al Jaber H, Nikolovski Z, Latiff A, Georgakopoulos C, Merenkov Z, Segura J, Alsayrafi M, Jaganjac M. Whole blood storage in CPDA1 blood bags alters erythrocyte membrane proteome. Oxid Med Cell Longev. 2018 (2018); 6375379.
6. Wishart S. Emerging applications of metabolomics in drug discovery and precision medicine. Nat Rev Drug Discov. 2016 Jul;15(7):473-84.

Uspješnost kolegija će evaluirati svake godine zajedničko stručno povjerenstvo Instituta Ruđer Bošković, Sveučilišta u Dubrovniku i Sveučilišta Josipa Jurja Strossmayera u Osijeku na temelju uspjeha na ispitu i anketa.