| Cipher: |
2304 |
| Nomenclature: |
Peroxidation of lipids in the basics of oxidative stress |
| Study programme: |
Molecular biosciences |
| Module: |
Biology |
| Case holder: |
Dr. sc. Sandra Sobočanec, Senior Research Associate
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| Institution of the case holder: |
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| Contributors - Contractors: |
dr.sc. Zlatko Marušić, zn. associate
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| Subject status: |
Electoral College |
| The year in which the case is submitted: |
Year I |
| The semester in which the case is submitted: |
Semester II |
| Subject objective: |
Understand the causal links of oxidative stress and the mechanism of lipid peroxidation process with the pathophysiology of various diseases and organic disorders.
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| Case contents: |
- free radicals and oxidative stress - definitions and examples
- reactive oxygen compounds (ROS) – formation and properties in biological systems
- lipid peroxidation, lipoproteins and reactive aldehydes
- oxidative stress and peroxidation of lipids in metabolic, inflammatory and degenerative diseases (diabetes, atherosclerosis, neurodegeneration, chronic renal failure, etc.)
- aging, oxidative stress and tumors
- oxidative stress as a link between hereditary and iyatrogenic tumors
- oxidative stress in carcinogenesis and tumour therapy
- methods for creating and measuring the reaction rate of free radicals
- detection of free radicals and other mediators of oxidation stress in biological systems
- chemical properties of ROS - reaction mechanisms and kinetics of chemical reactions
- oxidative stress and transition metals
- iron: a paradox in the field of oxygen radicals in biology
- iron metabolism - significance in oncology
- malignancies as systemic disorders, metabolic changes caused by tumors
- inflammation and tumours, the possible role of granulocytes in carcinogenesis and defence against tumours
- antioxidants
- carotenoid decomposition products and tumors
- strategies of antioxidant therapy in human diseases - facts and visions
- oxidative stress in lymphedema of surgically treated patients with tumors – products of lipid peroxidation in fibrosclerosis of lymphedema patients
- oxidative stress in clinical trials
- new diagnostic and therapeutic possibilities related to oxidative stress
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| Learning outcomes: competences, knowledge, skills that the subject develops: |
1. Determine the properties and reactions of free radicals in the basics of oxidative stress.
2. Predict the correlation between specific lipid peroxidation initiation mechanisms and potential organic disorders.
3. Review the methodology of researching the lipid peroxidation process with the aim of finding as direct a quantitative method of measuring the oxidation state as directly as possible
4. Evaluate the effectiveness of therapy or prevention by applying antioxidants.
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| ECTS Credits |
6 |
| Lectures |
5 |
| Seminars (IS) |
5 |
| Exercises (E) |
20 |
| Altogether |
30 |
| The way of teaching and acquiring knowledge: |
regular attendance with possible justified absence of up to 4 hours of classes
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| Ways of teaching and acquiring knowledge: (notes) |
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| Monitoring and evaluating students (mark in fat printing only relevant categories) |
Attendance |
| Rating method: |
Written exam |
| Mandatory literature: |
1. Halliwell, B.; Gutteridge, J.M.C. FREE RADICALS IN BIOLOGY AND MEDICINE. Oxford University Press, New York, 1999; Pp. 246-350.
2. Mihaljevic, B., Katusin-Razem, B., Razem, D. THE REEVALUATION OF THE FERRIC THIOCYANATE ASSAY FOR LIPID HYDROPEROXIDES WITH SPECIAL CONSIDERATIONS OF THE MECHANISTIC ASPECTS OF THE RESPONSE. Free Radical Biol. Med., 21:53-63; 1996
3. Žarković, N. MECHANISMUS DER TUMORENTSTEHUNG. Pharmazeutishe Zeitung, 145: 239-245; 2000
4. ŽARKOVIĆ, N. ANTIOXIDATIEN IN DER PRÄVENTION UND CHEMOTHERAPIE VON TUMOREN. Pharmazeutishe Zeitung, 145: 391-395: 2000
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| Supplementary (recommended) literature: |
1. Toyokuni, S.; Okamoto, K.; Yodoi, J.; Hiai, H.; PERSISTANT OXIDATIVE STRESS IN CANCER. FEBS Lett. 358: 1-3; 1995.
2. HuANG, X. IRON OVERLOAD AND ITS ASSOCIATION WITH CANCER RISK IN HUMANS: EVIDENCE FOR IRON AS A CARCINOGENIC METAL. Mutation Research, 533: 153-171: 2003.
3. Alia, A.J.; Bresgen, N.; Sommerburg, O.; Siems, W.; Eckl, P.M. GENOTOXIC EFFECTS OF BETA-CAROTENE BREAKDOWN PRODUCTS ON PRIMARY RAT HEPATOCYTES. Carcinogenesis 25, 827-831; 2004.
4. Schumacher M.; Eber B.; Tatzber F.; Kaufmann P.; Halwachs G.; Fruhwald F.M.; Zweiker R.; Esterbauer H.; Klein W. TRANSIENT REDUCTION OF AUTOANTIBODIES AGAINST OXIDIZED LDL IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION. Free Radic Biol Med 18:1087-1091; 1995
5. Tatzber, F.; Griebenow, S.; Wonisch, W.; Winkler R. DUAL METHOD FOR THE
DETERMINATION OF PEROXIDASE ACTIVITY AND TOTAL PEROXIDES – IODIDE LEADS TO A SIGNIFICANT INCREASE OF PEROXIDASE ACTIVITY IN
HUMAN SERA. Anal Biochem 316(2):147-153; 2003
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| How to monitor the quality and performance performance (evaluation): |
For individual lectures – thematic units - verification of understanding of the information obtained, the method of presentation, etc.
Discussions with students and colleagues - survey upon completion of the course.
Tracking each student's progress.
Evaluation of performance by the head of studies and the joint expert committee of the institutions of the holders of the study. The success of the course will be evaluated annually by the joint expert committee of the Rudjer Boskovic Institute, the University of Dubrovnik and the University of Osijek."
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